EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.12 | expressed in Escherichia coli M15rep4 cells | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.12 | C116S | MSRB1 mutant with decreased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme | Arabidopsis thaliana |
1.8.4.12 | C134T | MSRB2 mutant with increased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme | Arabidopsis thaliana |
1.8.4.12 | T132A | MSRB1 mutant with decreased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme | Arabidopsis thaliana |
1.8.4.12 | T132C | MSRB1 mutant with decreased kcat values for dabsyl-L-methionine (R)-sulfoxide compared to the wild type enzyme | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.12 | 0.0068 | - |
glutaredoxin C4 | isoform MSRB1 wild type enzyme | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.4.12 | plastid | - |
Arabidopsis thaliana | 9536 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.4.12 | 16610 | - |
reduced isoform MSRB1, MALDI-TOF mass spectrometry | Arabidopsis thaliana |
1.8.4.12 | 16620 | - |
reduced isoform MSRB1, calculated from amino acid sequence | Arabidopsis thaliana |
1.8.4.12 | 16860 | - |
reduced isoform MSRB2, calculated from amino acid sequence | Arabidopsis thaliana |
1.8.4.12 | 16860 | - |
reduced isoform MSRB2, MALDI-TOF mass spectrometry | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.12 | Arabidopsis thaliana | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.12 | HiTrap column chromatography and Sephadex G-25 gel filtration | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.12 | dabsyl-L-methionine (R)-sulfoxide + glutaredoxin C4 | - |
Arabidopsis thaliana | ? | - |
? | |
1.8.4.12 | dabsyl-L-methionine (R)-sulfoxide + glutaredoxin S12 | - |
Arabidopsis thaliana | ? | - |
? | |
1.8.4.12 | dabsyl-L-methionine (R)-sulfoxide + thioredoxin | - |
Arabidopsis thaliana | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
1.8.4.12 | dabsyl-L-methionine (R)-sulfoxide + thioredoxin h1 | - |
Arabidopsis thaliana | ? | - |
? | |
1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | - |
Arabidopsis thaliana | L-methionine + thioredoxin disulfide + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.12 | methionine sulfoxide reductase B | - |
Arabidopsis thaliana |
1.8.4.12 | MSRB1 | isoform | Arabidopsis thaliana |
1.8.4.12 | MsrB2 | isoform | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.12 | 0.002 | - |
dabsyl-L-methionine (R)-sulfoxide | isoform MSRB1 wild type enzyme, in the presence of 20 mMTris(2-carboxyethyl) phosphine hydrochloride | Arabidopsis thaliana | |
1.8.4.12 | 0.005 | - |
dabsyl-L-methionine (R)-sulfoxide | isoform MSRB1 mutant T132C, in the presence of 20 mM dithioerythritol | Arabidopsis thaliana | |
1.8.4.12 | 0.012 | - |
dabsyl-L-methionine (R)-sulfoxide | isoform MSRB1 mutant T132A, in the presence of 20 mM dithioerythritol | Arabidopsis thaliana | |
1.8.4.12 | 0.014 | - |
dabsyl-L-methionine (R)-sulfoxide | isoform MSRB2 wild type enzyme, in the presence of 20 mMTris(2-carboxyethyl) phosphine hydrochloride | Arabidopsis thaliana | |
1.8.4.12 | 0.02 | - |
glutaredoxin C4 | isoform MSRB2 wild type enzyme | Arabidopsis thaliana | |
1.8.4.12 | 0.028 | - |
dabsyl-L-methionine (R)-sulfoxide | isoform MSRB2 wild type enzyme, in the presence of 20 mM dithioerythritol | Arabidopsis thaliana | |
1.8.4.12 | 0.03 | - |
glutaredoxin S12 | isoform MSRB2 wild type enzyme | Arabidopsis thaliana | |
1.8.4.12 | 0.047 | - |
dabsyl-L-methionine (R)-sulfoxide | isoform MSRB1 mutant C116S, in the presence of 20 mM dithioerythritol | Arabidopsis thaliana | |
1.8.4.12 | 0.064 | - |
dabsyl-L-methionine (R)-sulfoxide | isoform MSRB2 mutant C134T, in the presence of 20 mM dithioerythritol | Arabidopsis thaliana | |
1.8.4.12 | 0.075 | - |
dabsyl-L-methionine (R)-sulfoxide | isoform MSRB1 wild type enzyme, in the presence of 20 mM dithioerythritol | Arabidopsis thaliana | |
1.8.4.12 | 0.3 | - |
glutaredoxin C4 | isoform MSRB2 mutant enzyme C134T | Arabidopsis thaliana | |
1.8.4.12 | 0.31 | - |
glutaredoxin S12 | isoform MSRB1 mutant enzyme T132A | Arabidopsis thaliana | |
1.8.4.12 | 0.34 | - |
glutaredoxin C4 | isoform MSRB1 mutant enzyme C116S | Arabidopsis thaliana | |
1.8.4.12 | 0.36 | - |
glutaredoxin S12 | isoform MSRB2 mutant enzyme C134T | Arabidopsis thaliana | |
1.8.4.12 | 0.37 | - |
glutaredoxin C4 | isoform MSRB1 mutant enzyme T132A | Arabidopsis thaliana | |
1.8.4.12 | 0.48 | - |
glutaredoxin C4 | isoform MSRB1 wild type enzyme | Arabidopsis thaliana | |
1.8.4.12 | 0.5 | - |
glutaredoxin S12 | isoform MSRB1 mutant enzyme C116S | Arabidopsis thaliana | |
1.8.4.12 | 0.58 | - |
glutaredoxin S12 | isoform MSRB1 wild type enzyme | Arabidopsis thaliana | |
1.8.4.12 | 1.28 | - |
thioredoxin h1 | isoform MSRB2 wild type enzyme | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.12 | thioredoxin | - |
Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.12 | 73.4 | - |
glutaredoxin C4 | isoform MSRB1 wild type enzyme | Arabidopsis thaliana |